Abstract
The activity of membrane proteins and compounds that interact with the membrane is modulated by the surrounding lipid composition. However, there are no simple methods that determine the composition of these annular phospholipids in eukaryotic systems. Herein, we describe a simple methodology that enables the identification and quantification of the lipid composition around membrane-associated compounds using SMA-nanodiscs and routine 1H-31P NMR.
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CITATION STYLE
Medina-Carmona, E., Varela, L., Hendry, A. C., Thompson, G. S., White, L. J., Boles, J. E., … Ortega-Roldan, J. L. (2020). A quantitative assay to study the lipid selectivity of membrane-associated systems using solution NMR. Chemical Communications, 56(78), 11665–11668. https://doi.org/10.1039/d0cc03612a
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