DeTERMINAÇÃO de citrato de sildenafila e de tadalafila por cromatografia liquida de ultraeficiência com dETECÇão por arranjo de diodos (CLUE-DAD)

Abstract

A simple ultra-performance liquid chromatographic method for the simultaneous determination of sildenafil citrate and tadalafll was developed and validated. Sample preparation was dissolution in methanol, followed by centrifugation and dilution (1:10) with methanol. Analysis was performed in an Acquity® UPLC system, with Acquity®BEH C18 column (2.1 × 50 mm, with 1.7 μm particles). The elution was isocratic with phosphate buffer pH 2.3 and acetonitrile (65:35, v/v) at a flow rate of 0.7 mL/min. The method, presented adequate specificity, linearity, precision and accuracy and allowed, the determination of the drugs in seized, forensic samples.