Chemical and Biological Profiling of Three Ferulic Acids Alkyl Esters Isolated from Jatropha pandurifolia (Family: Euphorbiaceae) Stem Bark

Abstract

The study's objectives include phytochemical profiling and biological (antioxidant, thrombolytic and cytotoxic) analysis of pure chemicals from Jatropha pandurifolia stem bark ethyl acetate extract. Five different compounds including octacosanyl cis ferulate (1), hexacosyl (E)-ferulate (2) triacontyl ferulate (3), ß-sitosterol (4) and stigmasterol (5) are elucidated. Their structures determine through 1HNMR analysis and comparison to published data, while three ferulic acid alkyl esters (1-3) were isolated for the first time from J. pandurifolia. Compounds 1, 2, and 3 all have significant thrombolytic potential with respective values of 68.92% ±1.17 (∗∗P<0.01), 66.56% ±2.35 (∗∗P<0.01) and 70.81%±0.98 (∗∗P<0.01) with comparison to standard streptokinase (73.6%±0.76). When compared to BHT (6.82± 0.99 μg/ml) the IC50 (DPPH assay) values were 16.26±1.07 (∗∗P<0.01), 14.12±1.23 (∗∗P<0.01), and 13.16±1.70 μg/ml (∗∗P<0.01). Comparing the three compounds to the reference vincristine sulphate (LC50: 0.52±0.18 μg/ml), of compound 1 (1.56±0.35 μg/ml) (∗∗P<0.01), compound 2 (1.3±0.78 μg/ml) (∗∗P<0.01) and compound 3 (1.29±0.33 μg/ml) (∗∗P<0.01). The results can therefore be interpreted as a concept of isolated molecules having potential for application in additional pharmaceutical research.