Haematological, Biochemical And Serum Electrolyte Changes In Non-Pregnant Boer Does Inoculated With Corynebacterium Pseudotuberculosis Via Various Routes

Abstract

Caseous lymphadenitis is a chronic disease characterized by internal and external abscesses and is caused by corynebacterium and pseudotuberculosis.Thisstudy is designed to measure the hematological, biochemicaland serum electrolyte changes in experimental non-pregnant doesinoculated withcorynebacterium pseudotuberculosisvia variousroutes. Little is known about the changes in these parameters through different routes of infection.A total of 20 healthy does (n=20) were divided into 4 groups (intradermal, intranasal, oral and control) of5 goats each. The three groups were inoculated with 10 7 cfu/1ml of live corynebacterium pseudotuberculosis, while control is kept unexposed. Following infection, blood samples were collected from the jugular veinfor hematological, biochemical and serum electrolyteanalysis. A significantdecrease was observed in RBC count (p<0.05) in the intradermal group, whilenochangesobservedin PCV, Hb, MCV and MCHC parameters. Significant increasein WBC were observed in intradermal, intranasal and oral groups (p<0.05). Slight increase (p<0.05) in monocyte count was observed in intranasal group. A significant reduction (p<0.05) in lymphocytic count was observed for the intranasally inoculated group(p<0.05) and a slight increase (p<0.05) in neutrophil was observed in intranasal and intradermal groups. Biochemically, decrease in albumin, increase in creatininelevelsinintranasal group(p<0.05) and GGT levels were elevated in all the three infected groups (p<0.05).However,there were no significant changes in AST, T.Protein and APT. Serumelectrolyte, revealed a decrease in Calcium (Ca +) concentration in intradermal group with concentration of 2.22mmol/L,intranasal 2.23mmol/L group (p<0.05), and no changes were observed in potassium (K +) and sodium (Na +). The study, therefore, observed increase in WBCs, neutrophils, monocytes, creatinine, GGT, levels and decrease in RBCs, lymphocytes, albumin and calcium concentrations on different route of infections.