Analisis Perbandingan HPLC dan Teknik Lain untuk Deteksi Antibiotik

Abstract

Antibiotics are a therapy used in bacterial infections. The β-lactam group, such as penicillin, amoxicillin and cephalosporin are first generation antibiotics that can be used in treating bacterial infections. Antibiotics can be analyzed using High Performance Liquid Chromatography (HPLC). Ultra high performance liquid chromatography (UHPLC) and high pressure liquid chromatography (HPLC) are important analytical techniques in molecular separation. UHPLC is specifically designed for higher pressures during chromatographic analysis with short columns and small particle sizes, while HPLC aims to separate molecules in minimum time. Nonetheless, method transfer and revalidation between UHPLC and HPLC is quite easy and can save time. The use of these liquid chromatography techniques allows for more efficient and time-saving analysis. In performing routine HPLC analysis, it is important to consider speed, sensitivity, resolution, cost of analysis, and column maintenance. Therefore, modern developments in liquid chromatography are applied to save time and solvent consumption. Since 2004, UHPLC has repeatedly demonstrated significant advantages over HPLC-based methods. Parameters used in method data validation include precision, accuracy, coefficient of variation, limit of detection (LOD), and limit of quantity (LOQ).