Effect of different organic waste on cellulose-degrading enzymes secreted by Petriella setifera in the presence of cellobiose and glucose

Abstract

Abstract: Cellulose degradation is less known in the Ascomycota phylum, but it is important to recognize it because this process influences the most important biochemical cycle: the carbon cycle. Cellulose degradation is carried out by a complex enzyme: cellulase. Petriella setifera has recently been recognized as a producer of cellulolytic enzymes. In this work, it was shown, that the activity of cellulose-degrading enzymes, carbon source utilization and the gene expression of P. setifera were not determined by pre-culturing in different lignocellulosic wastes. Moreover, it was found that the presence of glucose and cellobiose could inhibit the activity and the expression of the cellobiohydrolase enzyme. β-glucosidase exhibits a higher activity in all of the analysed wastes and this behaviour can be explained as a mechanism for P. setifera to overcome cellobiose-glucose inhibition through the transglycosylation reaction. The significance of our research is to enable researchers to understand more thoroughly the cellulose degradation caused by soft rot fungus which belongs to the Ascomycota phylum. Our research is contributing to the understanding of cellulase activity and gene expression inhibition through the availability of a carbon source for P. setifera. Furthermore, it facilitates the acquisition of more information concerning the fungus P. setifera. Graphic abstract: [Figure not available: see fulltext.].