Protein identifications on O'Farrell two-dimensional gels: Locations of 55 additional Escherichia coli proteins

Abstract

The resolution of proteins from whole-cell homogenates by two-dimensional gel electrophoresis is sufficiently reproducible and precise to permit different laboratories to exchange information about them. To the previous total of 81 the authors add the locations of 55 E. coli proteins determined with the aid of purified proteins and mutant strains supplied by many investigators. The criteria used to establish the identifications of protein spots include migration with marker proteins, altered position or amount in appropriate mutant or plasmid-carrying strain, physiological behavior, and peptide map pattern.