In vitro antifungal activity of aegle marmelos, syzygium cumini and pongamia pinnata extracts against fusarium oxysporum f. sp. cicero

Abstract

The present investigation was carried out using acetone, ethanol, methanol and chloroform extracts of different plants and bio agents against Fusarium oxysporum f. sp. ciceri. Effect of plant extracts and bio agents alone and in combination on wilt incidence of chickpea was assessed using pot culture method. Methanol extracts of Aegle marmelos was found most active (88.64%) followed by methanol extract of Pongamia pinnata (85.30%). Ability of these four solvents to produce maximum extraction yield from four different plants was also evaluated. Methanol extracts of A. marmelos at concentrations of 250, 500, 750 and 1000 μl was found compatible with Trichoderma viride and Pseudomonas fluorescens. Methanol also gave the highest percent extraction yield. Seed treatment with P. fluorescens 10 g/kg seed+T. viride 4 g/kg seed+methanol extract of A. marmelos 4% proved effective to reduce incidence of chickpea wilt disease caused by F. oxysporum f. sp. ciceri (69.31%).