A procedure for the isolation of deoxyribonucleic acid from micro-organisms

Abstract

A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA. Alternative methods of cell disruption and DNA isolation have been described and compared. DNA capable of transforming homologous strains has been used to test various steps in the procedure and preparations have been obtained possessing high specific activities. Representative samples have been characterized for their thermal stability and sedimentation behaviour. © 1961, Academic Press Inc. (London) Ltd.. All rights reserved.