Cannabinoid type 1 receptor is undetectable in rodent and primate cerebral neural stem cells but participates in radial neuronal migration

Abstract

Cannabinoid type 1 receptor (CB1 R) is expressed and participates in several aspects of cerebral cortex embryonic development as demonstrated with whole-transcriptome mRNA sequencing and other contemporary methods. However, the cellular location of CB1 R, which helps to specify molecular mechanisms, remains to be documented. Using three-dimensional (3D) electron microscopic reconstruction, we examined CB1 R immunolabeling in proliferating neural stem cells (NSCs) and migrating neurons in the embryonic mouse (Mus musculus) and rhesus macaque (Macaca mulatta) cerebral cortex. We found that the mitotic and postmitotic ventricular and subventricular zone (VZ and SVZ) cells are immunonegative in both species while radially migrating neurons in the intermediate zone (IZ) and cortical plate (CP) contain CB1 R-positive intracellular vesicles. CB1 R immunolabeling was more numerous and more extensive in monkeys compared to mice. In CB1 R-knock out mice, projection neurons in the IZ show migration abnormalities such as an increased number of lateral processes. Thus, in radially migrating neurons CB1 R provides a molecular substrate for the regulation of cell movement. Undetectable level of CB1 R in VZ/SVZ cells indicates that previously suggested direct CB1 R-transmitted regulation of cellular proliferation and fate determination demands rigorous re-examination. More abundant CB1 R expression in monkey compared to mouse suggests that therapeutic or recreational cannabis use may be more distressing for immature primate neurons than inferred from experiments with rodents.