Crustin-capped selenium nanowires against microbial pathogens and Japanese encephalitis mosquito vectors – Insights on their toxicity and internalization

Abstract

Herein, we reported a method to synthesize selenium nanowires (Cr-SeNWs) relying to purified cysteine-rich antimicrobial peptide crustin in presence of ascorbic acid. Cr-SeNWs were characterized by UV–vis, XRD, FTIR and Raman spectroscopy, as well as SEM, HR-TEM and EDAX. The UV–vis spectroscopy peak was noted at 350 nm. XRD showed the crystalline nature of Cr-SeNWs through diffraction peaks observed 2θ at 12° and 28° corresponding to (020), and (241) lattice planes, respectively. HR-TEM results shed light on the size of Cr-SeNWs, ranging from 17 to 47 nm. Raman spectroscopy and EDAX analysis of Cr-SeNWs showed presence of 57% selenium element. Furthermore, Cr-SeNWs showed higher antimicrobial activity on Gram-positive bacteria (Staphylococcus aureus, Enterococcus faecalis) over Gram-negative ones (Pseudomonas aeruginosa, Escherichia coli). The zone of inhibition was larger on S. aureus (50 μg/ml = 4.0 mm, 75 μg/ml = 7.2 mm) and E. faecalis (50 μg/ml = 3.1 mm, 75 μg/ml = 5.1 mm), over P. aeruginosa (50 μg/ml = 2.1 mm, 75 μg/ml = 4.8 mm), E. coli (50 μg/ml = 1.3 mm, 75 μg/ml = 4.3 mm) bacteria. The antibiofilm activity of Cr-SeNWs was also investigated and biofilm reduction was observed at 75 μg/ml. In addition, Cr-SeNWs were highly effective as larvicides against Zika virus and Japanese encephalitis mosquito vectors, i.e., Culex quinquefasciatus and Culex tritaeniorhynchus, with LC50 values of 4.15 and 4.85 mg/l, respectively. The nanowire toxicity and internalization was investigated through confocal laser scanning microscopy and histological studies. To investigate the potential of Cr-SeNWs for real-world applications, we also evaluated Cr-SeNWs in hemolytic assays, showing no cytotoxicity till 5 mg/ml. Besides, higher antioxidant activity at the concentration at 100 μg/ml was noted, if compared with purified crustin. The strong antioxidant potential of this nanomaterial can be helpful to boost the shelf-life potential of Cr-SeNWs-based pesticides and antimicrobials.