Abstract
A multiplex reverse transcriptase (RT)-PCR assay was developed for the simultaneous detection of three different fish viruses: infectious pancreatic necrosis virus (IPNV), infectious hematopoietic necrosis virus (IHNV), and viral hemorrhagic septicemia virus (VHSV). The sensitivity levels of the multiplex RT-PCR assay were 100, 1, and 32 50% tissue culture infective doses/ml for IPNV, IHNV, and VHSV, respectively.
Cite
CITATION STYLE
Williams, K., Blake, S., Sweeney, A., Singer, J. T., & Nicholson, B. L. (1999). Multiplex reverse transcriptase PCR assay for simultaneous detection of three fish viruses. Journal of Clinical Microbiology, 37(12), 4139–4141. https://doi.org/10.1128/jcm.37.12.4139-4141.1999
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