TerapannyaLimbah Kulit Buah Sawo Manila(Manilkara zapota) sebagai Anti Kandidiasis

Abstract

Abstrak Diabetes mellitus dapat memicu infeksi, salah satunya kandidiasis yang disebabkan Candida albicans. Pengobatan kandidiasis dengan antibiotik dapat menyebabkan resistensi, sehingga perlu alternatif bahan alam yang berfungsi sebagai antifungi, salah satunya sawo manila (Manilkara zapota). Kulit buah sawo manila belum banyak dimanfaatkan, sehingga perlu dilakukan pemanfaatan limbah kulit buah sawo manila. Tujuan penelitian untuk mengetahui aktivitas antifungi ekstrak kulit buah sawo manila terhadap Candida albicans. Pembuatan ekstrak menggunakan metode maserasi dengan pelarut etanol 70%. Ekstrak kulit buah sawo manila dilakukan skrining fitokimia senyawa metabolit sekunder, dan uji aktivitas antifungi dengan metode difusi sumuran. Ekstrak kulit buah sawo manila mengandung flavonoid, alkaloid, dan tanin. Pengujian antifungi kulit buah sawo manila pada konsentrasi ekstrak 30%, 40%, 50% dan 60% berturut-turut menghasilkan rata-rata diameter zona hambat sebesar 3,827 ± 0,947 mm; 5,460 ± 0,337 mm; 6,673 ± 0,351 mm; 8,053 ± 0,720 mm. Ekstrak kulit buah sawo manila memiliki aktivitas antifungi terhadap Candida albicans. Abstract Diabetes mellitus can trigger infections, one of which is candidiasis caused by Candida albicans. Candidiasis treatment with antibiotics can cause resistance, so it is necessary to use alternative natural ingredients that function as antifungals, one of which is manila sapodilla (Manilkara zapota). The peel of the manila sapodilla fruit has not been widely used, so it is necessary to utilize the waste of the manila sapodilla fruit peel. The aim of the study was to determine the antifungal activity of the extract of the manila sapodilla fruit peel against Candida albicans. Extracts were made using maceration method with 70% ethanol as solvent. Manila sapodilla peel extract was screened for phytochemical compounds of secondary metabolites, and antifungal activity was tested by well diffusion method. Manila sapodilla fruit peel extract contains flavonoids, alkaloids, and tannins. Antifungal testing of the peel of the sapodilla manila fruit at extract concentrations of 30%, 40%, 50% and 60% respectively resulted in an average inhibition zone diameter of 3.827 ± 0.947 mm; 5.460 ± 0.337 mm; 6.673 ± 0.351 mm; 8.053 ± 0.720 mm. Manila sapodilla fruit peel extract has antifungal activity against Candida albicans.