Determination of Selected Endocrine Disrupter Compounds at Trace Levels in Sewage Sludge Samples

Abstract

Simultaneous determination of endocrine disrupter compounds (EDCs), namely diltiazem, progesterone, benzylbutylphthalate (BBP), estrone, and carbamazepine (Cbz) were performed by using high performance LC-electrospray tandem MS. The ultrasound-aided sequential extraction of sludge samples was optimized to increase extraction efficiencies of the analytes; ranging between 93.0-98.3% recovery. The limit of detection values for diltiazem, progesterone, BBP, estrone, and Cbz were found as 0.78, 0.72, 0.24, 0.75, and 0.72μg/kg, respectively. Sludge samples taken from Ankara Tatlar; Hurma, Lara and Kemer of Antalya, and Middle East Technical University-vacuum rotating membrane wastewater treatment plant (WWTP) aeration tanks were analyzed for their EDCs contents under optimized conditions. Diltiazem was found in all the samples in the range between 116.4-180.8ng/g while progesterone and estrone were not detected in any of the samples analyzed with the exception of Tatlar WWTP. The BBP concentration was between beyond detection and 9195.5ng/g. In addition, Cbz was found in all the samples ranging from 25.6 to 118.8ng/g. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.