Basophil effector function and homeostasis during helminth infection

Abstract

Basophils are effector cells of the innate immune system that are associated with allergic inflammation and infections with helminth parasites. However, their development and in vivo functions are largely unknown. Here, we characterize basophil development, turnover, tissue localization, and effector function during infection with the helminth Nippostrongylus brasiliensis. Our results demonstrate that under homeostatic conditions ba-sophils have a lifespan of about 60 hours.N brasiliensis-induced basophilia is caused by increased de novo production of basophils in the bone marrow. Ba-sophils were found near the marginal zone in the red pulp of the spleen, in the lamina propria of the small intestine, and in the lung parenchyma. Activated ba-sophils promoted systemic eosinophilia,were associated with differentiation of alternatively activated macrophages in the lung, and contributed to efficient worm expulsion, demonstrating that basophils play a crucial role as effector cells in type 2 immune responses. © 2009 by The American Society of Hematology.