STUDYING THE EFFECTS OF SOME COMPOUNDS AND IONS ON Escherichia coli ALKALINE PHOSPHATASE ACTIVITY

Abstract

The effect of some compounds and ions such as EDTA,Urea,KCN,2-Mercaptoethanol,BeCl 2 and L-Phenyl alanine on the activity of Alkaline phosphatase extracted from Escherichia coli has been studied by using p-NitroPhenyl Phosphate as a substrate at a different concentrations (1.5×10-1 ,3.0×10-2 ,4.5×10-3) M.,and the alkaline glycine regulating solution pH=10.5.Inhibition constant K i and type of inhibition(competitive or noncompetitive)using Dixon plot for competitive & noncompetitive inhibition as well as the percentage of inhibition have all been calculated & I 50 for different compounds.