Toxic effect of different lead concentrations on in vitro culture of Datura inoxia

Abstract

Metals are the part of natural constituents of soil but industrial activities such as mining and smelting of ores, electroplating work, gas exhaust, energy and fuel production, fertilizer and pesticide application, and municipal waste results in enormous amount of increase in their natural concentrations in soil. Hazardous compounds or chemicals such as heavy metals, oils and battery metals from industries and organic solvents are major soil pollutants. These substances get deposited to the soils of the neighboring area and pollute them by changing the chemical and biological properties of soil of that area. The aim of this research was to identify some interesting accumulators which may associate an important biomass production with an effective heavy metal accumulation, absorption and translocation and their tissue culture approach. This research is mainly focused on the ability of some native plants to accumulate and tolerate high concentrations of heavy metals which are often associated in polluted areas. It has been found that Datura inoxia has the capacity to accumulate the heavy metals in its part. The in vitro culture of Datura inoxia shows better response in Lead supplementing M. S. Media. Although lead is a toxic heavy metal for plants, but Datura inoxia shows good response at higher concentration..