Prostaglandin production during spontaneous labor and after treatment with RU486 in pregnant rhesus macaques

Abstract

The production of prostaglandins from amnion, chorion, decidua, and myometrium was studied in a superfusion system to determine the level of prostaglandin production during late pregnancy, during spontaneous labor, and after in vivo treatment with RU486. Tissues were divided into three groups: those from pregnant control animals, those from animals receiving RU486 (20 mg/kg/day for 3 days) in vivo, and those from animals in spontaneous term labor. Tissues were collected at cesarean section and placed in the superfusion system. After a 30-min equilibration period, fractions of media were collected after passing through the tissue chambers and were assayed for prostaglandin F(2α) (PGF(2α)) and prostaglandin E2 (PGE2). The results showed a significant increase in PGF(2α) from decidua of animals treated with RU486 compared to pregnant controls (pregnant: 3.01 ± 0.45 ng/g/min; RU486: 4.45 ± 0.28 ng/g/min; p < 0.05). There was a dramatic increase in PGE2 production by amnion from animals in spontaneous labor but not after RU486 treatment (pregnant: 0.21 ± 0.06 ng/g/min; RU486: 0.56 ± 0.09 ng/g/min; spontaneous labor: 5.83 ± 0.43 ng/g/min; p < 0.01). We conclude that while progesterone is important for maintaining uterine quiescence during pregnancy, progesterone receptor blockade by RU486 does not lead to an increase in PGE2 production by amnion as is shown during normal spontaneous labor.