CircRIP2 promotes NSCLC progression by sponging for miR-671-5p to regulate FOXM1 expression

Abstract

Lot of attention had been paid to the role of circular RNAs (circRNAs) in carcinogenesis recently. However, knowl edge about ci rcRNAs i n NSCLC development is far from satisfactory. In this study, we aimed to provide a novel insight into the circRIP2 in NSCLC development. We used NSCLC tissues, as well as cell lines to elucidate the expression and location of circRIP2 in NSCLC. We also established the circRIP2 overexpression cells A549-circRIP2 and repression cells HCC827-shci r cRI P2 f or f ur t her f unct i onal and mechani sm st udi es. The pr o-t umor i geni c r ol e of ci r cRI P2 was t est ed by usi ng CCK-8, Br dU and transwell assays. The interaction between circRIP2 and miR-671-5p were validated by luciferase reporter assay, RIP assay, as well as RNA pull down assay. We showed circRIP2 is differentially expressed NSCLC, and acted as a predictor for overall survival (OS) and disease-free survival (DFS). CircRIP2 promoted NSCLC progression by acting as a miRNA sponge for miR-671-5p, thus facilitating its target gene FOXM1 expression. Targeting circRIP2 could be potentially beneficial for NSCLC patients in the future.