In Vivo Evaluation of Fine Needle Amperometric Glucose Sensors Implanted in Rabbit's Blood Vessel

Abstract

Fine needle amperometric glucose sensors having outer membrane of heparin in addition of polyurethane/polydimethylsiloxane film were prepared and their glucose sensor properties were evaluated in phosphate buffer solution and in vivo monitoring by the implantation of the sensor in veins of rabbit ears. The sensitivity of the sensor was 2.2 mu A mM(-1) cm(-2) and the detection limit for glucose was 77 mu M (based on S/N = 3). Glucose sensors without heparin membrane showed glucose response on the day of implantation in vein, while reliable response was not obtained on the second day. On the other hand, the sensor with heparin outer membrane provided clear glucose response also after two days of implantation. This result indicated that the introduction of heparin membrane increased the biocompatibility of the sensor and improved the stability of sensor in vein. (C) 2013 The Electrochemical Society. All rights reserved.