Dicalcium phosphate (CaHPO4·2H2O) precipitation through ortho- or meta-phosphoric acid-etching: Effects on the durability and nanoleakage/ultra-morphology of resin-dentine interfaces

Abstract

Objectives To compare the effects of two etching procedures using meta-phosphoric (MPA) or ortho-phosphoric acid (OPA) on dentine demineralisation, resin-dentine bonds durability and interface nanoleakage/ultra-morphology. Methods Middle-dentine specimens were etched using 37% OPA (15 s) or 40% MPA (60 s) and submitted to infrared spectroscopy (FTIR) or ultra-morphology dye-assisted (calcium-staining) confocal microscopy (Ca-CLSM). A three-step etch-and-rinse adhesive was formulated, applied onto dentine and light-cured for 30 s before composite build-up. After 24 h, the dentine-bonded specimens were cut into 1 mm2 beams; half were immediately submitted to microtensile bond strength (μTBS) and half stored in DW for six months. The μTBS results were analysed with repeated-measures ANOVA and Tukey's test (p < 0.05). Further teeth were bonded and prepared for interface nanoleakage/ultra-morphology confocal evaluation. Results FTIR and Ca-CLSM analyses showed dicalcium phosphate dihydrate (Brushite) precipitation in MPA-etched dentine and on the bottom (front of demineralisation) of the OPA-etched dentine. Statistical analysis showed similar μTBS for both etching procedures after 24 h. The μTBS of specimens in OPA-group dropped significantly (p < 0.05) after six month; the specimens in the MPA group showed no statistically difference (p > 0.05). CLSM depicted no evident sign of nanoleakage within the resin-dentine interface of the MPA-treated specimens, while the specimens in OPA-group presented intense nanoleakage and interface degradation. Conclusion The use of MPA (60 s) as an alternative dentine conditioning agent in etch-and-rinse bonding procedures may be a suitable strategy to create more durable resin-dentine bonds. © 2013 Elsevier Ltd.