The effect of purification on Snail (Achatina fulica) cellulase enzyme characteristic

Abstract

Snail is an organism that able to produce cellulase enzyme due to its ability to use cellulose for energy source. The purposes of this study were to obtain best concentration of ammonium sulphate in increasing cellulase enzyme activity, optimum temperature, and cellulase enzyme specific substrate from snail. The snail cellulase enzyme was concentrated using ammonium sulphate. The study was designed as Completely Randomized Design consisting seven treatments (30-90% of ammonium sulphate) and three replicates. The result showed that the activity of crude cellulase enzyme was 0,123 U/ml. The optimum cellulose enzyme activity was at 50% ammonium sulphate as 0,609 U/ml and the specific activity was 0,970 U/mg. Optimum temperature of the concentrated cellulose enzyme was at 50°C and the highest activity was in CMC substrate at 0,396 U/ml.