The Use of Bio-Manufactured Nano-Zinc from Bacillus cereus in the Control of Dreshslera oryzae Fungus

Abstract

This research set out to create biosynthetic nano zinc ZnO-NPs by employing Bacillus cereus, characterize said nano zinc, and then test said nano zinc's ability to thwart the spread of Dreshslera oryzae, the fungus responsible for rice's brown spot disease. Bacillus cereus was used in a biological process to produce nano-sized zinc oxide, and this nano-sized zinc was then analyzed using a number of different methods. The ZnO-NPs generated from B. cereus had a nano zinc size of 22.48 nm, and their UV-Spectrophotometer examination indicated a significant absorption peak at 390 nm. Furthermore, the X-ray diffraction (XRD) results demonstrated that the particles are crystalline in nature. The Scanning Electron Microscope (SEM) analysis confirmed that the ZnO-NP produced by the B. cereus bacteria are well coordinated, mostly spherical, and somewhat homogenous in size (ranging from 37 nm). Taking a three-dimensional image with the atomic force microscopes also confirmed the surface form of the bacterial bio-zinc oxide nanoparticles (AFM). It was also determined that a concentration of 20 g/ml of nano zinc is optimal for preventing the growth of the fungus D. oryzae, which is responsible for the brown spot disease on rice. ZnO-NPs derived from B. cereus completely inhibited the growth of all of the tested fungal isolates, while the control treatment had no effect.