Isolation and characterization of CcAbf62A, a GH62 α-L- arabinofuranosidase, from the basidiomycete Coprinopsis cinerea

Abstract

A cDNA encoding a putative extracellular α-l-arabinofuranosidase was cloned from the basidiomycete Coprinopsis cinerea (CcAbf62A). CcAbf62A belongs to glycoside hydrolase family 62 (GH62), but is phyloge-netically distinct from previously characterized GH62 enzymes. The recombinant CcAbf62A, expressed in Pichia pastoris, released L-arabinose from both wheat arabinoxylan and oat-spelt xylan. The enzyme activity for wheat arabinoxylan was increased by the addition of CcEstl, a carbohydrate esterase from C. cinerea.