Analysis of eEF1Bγ interactome in the nuclear fraction of A549 human lung adenocarcinoma cells

Abstract

To study the translation elongation factor eEF1B gamma (eEF1Bγ) in the nucleus of lung carcinoma cells. Methods. The protein partners of eEF1Bγin the nuclear fraction of A549 cells were identified by co-immunoprecipitation (co-IP) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The protein interaction network for nuclear eEF1Bγ was determined by Cytoscape 3.2.0 program using the MCODE plugin. Additional analysis of the eEF1Bγ partners was conducted by Map of the cell database. Results. 234 proteins interacting with eEF1Bγ in the nuclear fraction of A549 cells were identified. Possible functional networks involving these contacts were analyzed by two bioinformatic approaches. Conclusions. Splicing of pre-mRNA and regulation of mRNA stability are assumed to be the main processes in which nuclear eEF1Bγ can be involved. We hypothesize that a portion of eEF1Bγ leaves the cytoplasm-localizede EF1B complex during carcinogenesis and enters the nucleus to regulate certain mRNAs by affecting the splicing of their pre-mRNA and/or stability of mRNA.