Description of a laccase gene from Pleurotus ostreatus expressed under submerged fermentation conditions

Abstract

In this work, a gene (lacP83) encoding a Pleurotus ostreatus laccase isoenzyme expressed in submerged fermentation conditions is described. A 2,887 bp sequence was obtained from a genomic library of P. ostreatus by using a PCR inverse strategy. The coding sequence, 1,527 bp long, showed 17 exons and encoded a protein of 509 amino acids, with a putative signal peptide and conserved copper binding domains. The promoter region of the lacP83 gene (466 bp upstream of ATG) contains putative binding transcription factors such as MRE, XRE, a defense response element, and a stress response element. The protein and gene sequences of lacP83 showed, respectively, 90 to 96% and 78 to 92% of similarity to laccases of Pleurotus previously reported. However, it showed differences in its apparent molecular weight and promoter sequence.