Direct organogenesis of Passiflora foetida L. through nodal explants

Abstract

Passiflora foetida L. belongs to the family Passiflroaceae, one of the best known remedies for asthma and biliousness, is cultivated in different parts of India. It was regenerated from nodal explants through direct organogenesis without intervening callus phase. High frequency multiple shoots were formed on MS supplemented with 2.0 mg/l BAP + 1.0 mg/l Kn with 85% of survival rate. Maximum range of shoot elongation was successfully obtained on MS with 1.5 mg/l BAP + 0.5 mg/l NAA. The best root induction was found on 0.5 mg/l NAA + 0.5 mg/l IBA. This protocol seems to have enough potentiality for micropropagation of this valuable germplasm.