Prevalence of Pathogenic Bacterial Isolates Infecting Wounds and their Antibiotic Sensitivity

Abstract

Wound infection is one of the health problems that are caused by pathogenic bacteria and antibiotics resistance profiles of Gram negative bacteria increased treatment cost especially in diabetic patients. Thus, the objective of this work was to isolate, purification and identification of pathogenic bacteria from wound infection then determination of susceptibility testing against antibiotics. 41 isolated from wound infections were collected from microbiology laboratory of Zagazig University Hospitals and some private microbiology laboratory Cairo, Egypt. Bacterial isolates were identification by API 20E Enterobacteriaceae. These pathogenic bacterial isolates belong to these genera (Klebsiella, Enterobacter, Pseudomonas, Providencia, Serratia and Citrobacter). The frequency of the bacterial isolates was 24%, 20%, 14%, 12%, 6% and 6% of the bacterial pathogens isolates in this study respectively. Finally, the antimicrobial susceptibility was performed by disc diffusion method against 12 types of antibiotics covered all mode of action of bacteria.