Presence of putative sphingomyelinase genes among members of the family Leptospiraceae

Abstract

The presence of multiple DNA elements in pathogenic members of the family Leptospiraceae, similar to the sphA sphingomyelinase gene from Leptospira borgpetersenii, was demonstrated by low-stringency hybridization experiments. These DNA elements were designated putative sphingomyelinase genes. Grouping of strains by similarity of hybridization patterns corresponds to the species subdivision of the family Leptospiraceae on the basis of genetic characteristics. Therefore, hybridization with the sphA gene can be used as a taxonomic tool. These hybridization experiments indicate the presence of two groups of genetically related pathogenic Leptospira species.