High-performance liquid chromatographic analysis of the isomerization products of carbohydrates

Abstract

A high-performance liquid chromatographic system for the separation of complex mixtures of carbohydrates is described. The efficiency of the system is based on the application of anion-exchange resins (particle size 10-20 μm), a specially designed gradient chamber and a solution of orcinol in concentrated sulphuric acid as the detection reagent. On the basis of the chromatographic separations, a reaction mechanism for the molybdic acid-catalyzed isomerization of aldopyranoses is given. © 1976.