Influence of transfection process on single cell impedance

Abstract

Many new therapeutic approaches are based on gene transfection of cells. Data about dynamic interaction with cell membranes can support the selection of polymers and polyplexes regarding an optimal gene delivery reagent. A microchip/-fluidic system and an impedance-based method for monitoring the effects on individual cells after application of polymeric DNA-delivery systems is developed and evaluated. It was successfully demonstrated that the effects of polymers on the cell membrane can be monitored over time by using impedance measurements. © 2010 IOP Publishing Ltd.