Catalytic membrane with the recombinant catalase from psychrotolerant bacteria Serratia sp. in dairy applications

Abstract

The preparations of catalase immobilized into a membrane are a promising solution for the industrial application, for example, in dairy processes, in which hydrogen peroxide is used as a preservative agent. The activity of the immobilized recombinant catalase from psychrotolerant bacteria Serratia sp. confirms its huge application potential, since the preparation is active in cow milk solution (pH 6.6) and at low temperature (8°C–12°C). This work shows the results of above-mentioned catalase, chemically immobilized via divinyl sulfone on the regenerated cellulosic membrane. The immobilized enzyme exhibits kinetics in accordance with Michaelis–Menten equation until 1.8 and 2.5 g/L of H2O2, for 12°C and 8°C, respectively. Moreover, the obtained preparation keeps more than 50% of the initial activity for 3.5–6.6 h under process condition (1–2 g/L H2O2, pH 6.6, 8°C–12°C).