Analysis of alphalactalbumin and betalactoglobulin from the rehydration of bovine colostrum powder using cloud point extraction and mass spectrometry

Abstract

Alphalactalbumin (-La) and betalactoglobulin (-Lg) in the rehydration of bovine colostrum powder were successfully separated by cloud point extraction using a nonionic surfactant Triton X-114. The effects of different factors, including the surfactant concentration, sample volume, electrolyte, and pH were discussed. The optimized conditions for cloud point extraction of alphalactalbumin (-La) and betalactoglobulin (-Lg) can be concluded that the best surfactant is 1 (w/v) Triton X-114, 200 L of sample volume, 150 mmol/L NaCl, and 6 (w/v) sucrose. After cloud point extraction, the capillary electrophoresis is used to check the efficiency of the extraction procedure. The results had been effectively confirmed by the characterization with matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). Copyright 2012 Fan Zhang et al.