Recombinant Antibodies and Non-Antibody Scaffolds for Immunoassays

Abstract

The measurement of trace amounts of physiologically active small molecules (for example, lipids, drugs, other synthetic chemicals and metals) is critical for both clinical and environmental analyses. Most small molecules can be analyzed using highly sophisticated analytical techniques, including high pressure liquid chromatography (HPLC), gas chromatography (GC), and inductively coupled plasma atomic emission spectroscopy (ICPAES). However, these methods require extensive purification, experienced technicians, and expensive instruments and reagents. Immunoassays offer an alternative to these instrument-intensive methods. Immunoassays rely on an antibody (Ab), or mixture of antibodies, for recognition of the molecule being analyzed (the analyte). Immunoassays are frequently applied to the analysis of both low molecular ligands and macromolecular drugs, and are also applied in such important areas as the quantitation of biomarkers that indicate disease progression and immunogenicity of therapeutic drug candidates. The performance of immunoassays is critically dependent on the binding properties of the antibody used in the analysis, and identification of suitable antibodies is often a major hurdle in assay development. Recombinant antibodies will play a major role in future immunoassay development.