Efficiency of Trichoderma spp. Against of some pathogenic fungi causing of broad bean root rot disease

Abstract

This study aims to a survey broad bean root rot disease in Babylon governorate (80 km south Baghdad-Iraq), and evaluation the antagonistic activity of the biological control agents Trichoderma harzianium and T. viride against pathogens. The result showed the distribution of the disease in all fields in percentage of disease incidence 65-100% and severity 22.50-68.75%. Results of isolation and identification showed the presence of 6 species of fungi associated with infected plants with variable percentage of presence. Fusarium solani and Rhizoctonia solani were present in most samples, whereas other fungi Macrophomina phasealina, Aspergillus niger, Trichoderma sp. and Penecillium sp. were found with a low rate. T. harzianums and T. viride fungi had highly antagonistic ability against of pathogenic isolates, and protect broad bean plant from infection by pathogenic fungi by reduced diseases incidence and severity and increased plant growth promoting compared to control (with pathogen). The bio-control agents T. viride, T. harzianium alone increased plant length to38.67, 39.79 cm and fresh weight to24.33,24.76 g and dry weight to 2.00, 2.27g respectively compared to control.