Lipase production from thermophilic bacteria using waste frying oil as substrate

Abstract

Lipases are widely used in textile, food, medical and chemical industries. The present study was performed to produce lipase from thermophilic bacterial strains using waste frying oil as substrate. Among four bacterial strains, Bacillus licheniformis A7 (GenBank accesion number: KC310458 ) was determined to be the best lipase producer. A frying oil concentration of 40 mL/L, temperature of 55 °C, initial pH of 6.0 and incubation time of 72 h were found to be optimal for lipase production from Bacillus sp. Under the optimal culture conditions, the maximum cell growth and lipase activity were determined as 2.219 0D 600nm and 1607 U/L, respectively.