Researches on the Reproduction of “Ottoman Strawberry” with Tissue Culture

Abstract

Plant tissue culture is widely used as auxiliary methods for vegetative propagation due to give results in a shorter time and to obtain mass plant production than other vegetative propagation methods. The combined effect of BAP and IBA hormones (0.1 mg/l IBA; 0.2 mg/l IBA; 0.3 mg/l IBA; 0.5 mg/l BAP; 0.5 mg/l BAP + 0.1 mg/l IBA; 0.5 mg/l BAP + 0.2 mg/l IBA; 0.5 mg/l BAP + 0.3 mg/l IBA) was studied relative to average number of shoots per explant and root formation (%) of 42-day-old in strawberry cultivar "Ottoman Strawberry" shoot tip cuttings. Our results have shown that concentration of BAP + IBA (0.5+ 0.1 mg/l) in MS medium successfully resulted in the induction of average number of shoots and root formation per explant when compared to other treatment doses and control. Alone concentration of BAP had not significant effect on development of average number of shoots per explant and root formation. Development of average number of shoots per explant and root formation was inhibited as the BAP + IBA concentration increased or decreased except for MS culture medium 5 to 0.5 mg/l BAP and 0.1 mg/l IBA. Consequently, in strawberry cultivar "Ottoman Strawberry" tissue culture, application of alone BAP in the concentrations used is discouraged, while application of BAP + IBA (0.5 + 0.1 mg/l) concentrations is recommended.