Identification of Compounds Flavonoids Namnam Leaf Extract (Cynometra Cauliflora) As Inhibiting A-Glucosidase

Abstract

Plants namnam (Cynometra cauliflora) has the potential to be developed for a variety of bioactive ingredients in particular flavonoid compounds as inhibitors of α-glucosidase. These compounds can be extracted with various solvents and column chromatography, and can be also characterized by a variety of instruments. In this study, these compounds liquid-liquid extraction with n-butanol followed by column chromatography process. Meanwhile, characterization and separation of the most active fractions using TLC (Thin Layer Chromatography), FTIR (Fourier Transform Infrared) and LCMS (Liquid Chromatography Mass Spectrometry). Characterization of compounds with FTIR results show similarities with flavonoids for their -OH groups (u 3200-3450 cm-1), C = C aromatic (u 1500-1675 cm-1), -CH aliphatic (u 1350-1470 cm-1), -CO (u 1080-1300 cm-1), and C = O (u 1690-1766 cm-1). While these results Characterization using LCMS showed flavonoid compounds such as xanthotoxin, Fraxetin, capensine, naringenin, malvidin, cyanidin, amorphigenin, nobiletin, isorhamnetin, epigallocathecin, gallate, apigenin and oenin. The compounds are alleged to have inhibitory activity against α-glucosidase among fraxetin, oenin, naringenin, malvidin, and cyanidin. Thus, the plant leaves Namnam has the potential to be developed as a natural therapy of diabetes mellitus through the mechanism of inhibition of α-glucosidase.