Anti-Cancer Activity And Anti-Proliferation Ant Nests Flavonoid Fraction Test (Myrmecodya Pendans) Human Tongue Cancer Cells In Sp-C1

Abstract

Squamous cell carcinoma of the tongue is a malignant tumor derived from epithelial mucosa of the oral cavity and is largely a type of epidermoid carcinoma. The purpose of the study is to identify and analyze the effect of the flavonoid fraction of ant nests (Myrmecodia pendans) as an anti-cancer barrier against proliferation through Akt signaling and barrier mechanism of NF-KB in cancer cells SP-C1 of the tongue. The results of the study showed that the cytotoxicity test had the highest concentration of 1000 µg/ml to the lowest concentration of 7.8125 µg/ml in ethyl acetate fraction, ethanol, hexan fraction and water generates significant percentage data of the tongue cancer cell death SP-C1. Ethyl acetate flavonoid fraction in a concentration of 1000 µg/ml caused the percentage of cell death as much as 64.60%, and the lowest concentration of 7.8125 µg/ml led to cell death as much as 15.80 %. LC50 values of ethyl acetate fraction, ethanol fraction, hexan fraction and water fraction, respectively for 452.059 µg/ml; 937.562 µg/ml; 2691.535 µg/ml; 12302.69 µg/ml. Analysis antiproliferative flavonoid fraction and ethyl acetate fraction of ethanol based on concentration and incubation time to absorption optical density SP-C1 cells was statistically highly significant (p=0.00).