Estimation of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of Antimicrobial peptides of Saccharomyces boulardii against Selected Pathogenic Strains

Abstract

Saccharomyces boulardii is used for antimicrobial peptide production and so can be termed as bacteriocin molecules with probiotic potential. Antimicrobial peptides are extracted from the culture broth and followed the purification using Sephadex G-50 column. Further, the inhibition activity was determined against selected microorganisms and inhibition zone against streptococcus pneumoniae was found to be highest among selected microbes. The fourth fraction of the peptide after purification was shown a 26 mm bacterial inhibition zone determined by agar well diffusion method. SDS-PAGE analysis revealed the protein band corresponding to 18 kDa was observed. The MBC/MIC study proved that the peptide extract of S. boulardii has bactericidal activity.