Aquabiotechnology of directed cultivation of microalgae Chlorella sorokiniana biomass

Abstract

We carried out cultivation of Chlorella sorokiniana microalga using various irradiation sources: daylight lamp with spectral range 530-610 nm and filament lamp with spectral range 3.5-5.0 μm. Specific growth rates were evaluated for these conditions at exponential phase. From the obtained biomass we extracted lipids and determined its' fatty-acid content. Also we analyzed triacylglycerol (TAG) content and unsaturated fatty acids, as well as oleinic acid content. Biomass irradiation by infrared source causes enlarging of total lipid content in alga biomass, as well as saturated fatty acids and oleinic acid portions. It is viable to use infrared irradiation mode for accumulation of non-polar lipids (TAG) at stationary growth phase, which might be used for biofuel obtaining after fractionating. Polar lipid fraction, extracted from biomass at linear growth stage, might be used for obtaining essential polyunsaturated fatty acids.