Isolation of small protoplasts from Aspergillus niger.

Abstract

Isolation of small protoplasts from Aspergillus niger. A rider to be added is that we are isolating RNA from cultures growing under rather benign conditions. If cultures are grown under conditions likely to induce high levels of nucleases (e.g. phosphate or nitrogen limitation) then there may be problems with nucleases. In that case we stress the rapid freezing in liquid nitrogen and suggest homogenising the mycelium in a larger volume of urea/lithium chloride (say 25-40 vols) to dilute the nucleases.