Purification, crystallization and preliminary X-ray analysis of Hsp33 from Saccharomyces cerevisiae

Abstract

The heat-shock protein Hsp33 from the yeast Saccharomyces cerevisiae has been overexpressed, purified and crystallized. A crystal was obtained using the hanging-drop vapour-diffusion method and a data set was collected to 2.7 Å resolution. The crystal belongs to space group P43212, with unit-cell parameters a = b = 96.43, c = 132.22 Å, α = β = γ = 90°. The asymmetric unit is assumed to contain two subunits of Hsp33, with a V M value of 2.96 Å3 Da-1 and a solvent content of 58.41%. © International Union of Crystallography 2007.