Amplified detection of DNA through an autocatalytic and catabolic DNAzyme-mediated process

Abstract

Sense and sensibility: Amplified DNA detection is accomplished by the autocatalytic cleavage of a programmed nucleic acid structure based on the Mg2+-dependent DNAzyme E6 (see picture). The analyte sequence is autocatalytically generated by the DNAzyme from a reporter nucleic acid, which is labeled with a fluorophore and quencher at its two ends. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.