Metagenomic analysis of pathogen mastitis in cow's milk from Cicurug, Sukabumi, West Java, Indonesia

Abstract

Mastitis is a complex disease in cattle that it involves interactions between management practices and infectious agents. The common microorganisms causing mastitis are bacteria, besides this disease can be caused by mycoplasma, algae, and yeast. Pathogen microorganisms in milk can be obtained from cattle, human hands, equipment and the environment. This study aims to analyze the metagenomic of pathogen mastitis in cow's milk from Cicurug, Sukabumi, West Java. ZymoBIOMICSTM DNA Miniprep Kit was used for genome isolation to metagenomic analysis. The 16S rRNA PCR amplification was used for analysis the results of miniprep. Metagenomic analysis from subclinical mastitis milk showed that bacteria in cow's milk were the genera of Corynebacterium_1 (20.53 %), Corynebacterium (11.67%), Solibacillus (8.78%), Romboutsia (5.45%), Micrococcus (4.18%), Acinetobacter (3.64%), Aerosphaera (1.94%), Ignavigranum (1.90%), Lysinibacillus (1.49%), and Staphylococcus (1.38%).