The effects of flash-annealing on glycerol kinase crystals

Abstract

Reflection profiles from glycerol kinase crystals were analyzed to determine the effect of flash-cooling on mosaicity (η) and peak intensity in order to reveal changes in mosaic domain structure and composition. The results showed that repeated flash-annealing causes a significant decrease in the averaged mosaicity along with an increase in the overall peak counts of reflections and an enhanced signal-to-noise ratio. Individual reflection-profile analysis revealed a mostly dual domain structure, showing the minimization of one domain as a result of flash-annealing. © 2005 International Union of Crystallography - all rights reserved.