Preparation and Applicability of Sarcocystis cruzi Antigens and Their Anti-5. cruzi Rabbit Sera for Serodiagnosis of Bovine Sarcocystosis

Abstract

Sarcocystis cruzi antigens and their antisera were prepared for the seroimmunological diagnosis of bovine sarcocystosis. Fresh 5. cruzi cysts directly removed from cardial muscle of slaughtered cattle were digested with trypsin to release bradyzoites. By twelve cycles of freezing at-22¼C and thawing at 37¼C, bradyzoites were let to leach out soluble material. The soluble antigens were inoculated four times to rabbits at a dose of 343 μg protein and anti-5. cruzi sera were prepared with blood of the rabbits. Gel immunodiffusion test showed no cross-reaction between the present antigens and any of anti-Toxoplasma gondii, -Hammondia hammondi and - Besnoitia wallacei rabbit sera. Avidin-biotin complex immunoperoxidase (ABC) technique with the present anti-5. curzi rabbit sera showed clear positive reaction against 5. cruzi cysts in the muscular sections of infected cattle. © 1994, Japanese Society of Veterinary Science. All rights reserved.