Green approaches in sample preparation for the estimation of atenolol, aspirin, atorvastatin calcium and losartan potassium in bulk and its pharmaceutical dosage form prior to chromatographic technique

Abstract

Objective: A multivariate statistics method, simple, economical and eco friendly method for simultaneous estimation of Atenolol, Aspirin, Atorvastatin and Losartan potassium in bulk and its Pharmaceutical dosage form using liquid chromatographic method. Method: The method has been developed by using C18 column (250 • 4.6 mm, 5 lm) with UV detection at 237 nm. The developed method optimized by using Quality by design (QbD) technique and Derringer’s desirability function was applied. The sample solution is prepared by using dispersive liquid –liquid extraction (DLLME). Results: Central Composite Design was used to study the effect of independent factors. Derringer’s desirability function was applied to simultaneously optimize the retention time of last eluting peak (Atorvastatin calcium), capacity factor and resolution between Aspirin and Losartan potasium. The optimized method was validated according to ICH guidelines. The another important aspect is DLLME that the method uses microlitre amounts of organic solvent, produces low levels of waste and minimizing effluent treatment, which contributes to the environment and implements methods aimed green chemistry, making economic and environment safe for the people. Conclusion: The method can be employed as an alternative in quality control routine analysis of pharmaceutical industry for quantification of Atenolol, Aspirin, Atorvastatin and Losartan potassium in bulk and its Pharmaceutical dosage form using liquid chromatographic method.