Phytochemical and HPTLC studies of methanolic extract of Indigofera tinctoria (Fabaceae)

Abstract

Investigation on the chemical constituents including the chemical structure of major components in the water extracts from Strobilanthes flaccidifolius Nees. and Indigofera tinctoria Linn. was done. It was found that the indigo from fresh leaves of S. flaccidifolius Nees. fermented for three days gave the highest amount of indigo. The thin layer chromatography of the crude extract revealed four pigments; the major pigments were of red and blue having R f values of 0.88 and 0.76, respectively. The R f value, the maximum absorption from UV-Visible spectroscopy and infra red spectrum of blue pigment were the same as those of the standard indigo. Both blue and red pigments are highly soluble in chloroform whereas only red pigment is soluble in methanol.