The viability test of sappan wood (Caesalpinia sappan L.) ethanol extract in the H9C2 cell line

Abstract

Objective: In this study, the embryonic rat cardiomyocyte cell line H9C2 was used to investigate the cardiotoxicity effect of sappan wood ethanol extract (SWEE). Methods: Sappan wood was extracted in 96% ethanol and divided into dose concentrations of 2.5, 5, 10, 50, 100, and 150 µg/ml, with deferiprone used as a control. Cell viability was assessed using the PrestoBlue Cell Viability Reagent, according to manufacturer protocols. Results: Microscopic examination showed that the cell viability of H9C2 was preserved by SWEE treatments at a dose of 10 µg/ml and suggested dose concentrations of 50 µg/ml of SWEE. The percentage of viable cells was greater than 95% with a dose concentration of 10 µg/ml of SWEE, but it was significantly reduced with a dose concentration of 50 µg/ml of SWEE (p<0.05). Conclusion: The optimal dose concentration of SWEE to reach 95% cell viability was 10 µg/ml.